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KMID : 0350519940470010443
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Journal of Catholic Medical College
1994 Volume.47 No. 1 p.443 ~ p.455
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Effects of Multi-Iamellar Vesicles on the Disruption of Stratum Corneum Lipids Barrier in Hairless Mice
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Abstract
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Stratum corneum lipids play an important role in the permeability barrier and water content of the skin. Its water-holding function mainly depends on the lamellar structure composed of these lipids but it is still not well-known whether this
structure
is correlated with permeability barrier.
In order to investigate the effect of multi-lamellar vesicles having analogy to lamellar structure of stratum corneum lipids on the disruption of stratum corneum lipids barrier induced by acetone, permeability barrier, water content and
morphologic
changes of stratum corneum lipids were evaluated in the hairless mice skin under various conditions including distilled water(D/W)-treated control(group I), application of acetone( group Ⅱ), application of acetone & multi-lamellar vesicles
(group
Ⅲ),
& application of acetone & vehicle(group Ⅳ) for the air-exposed groups and control (group V), application of acetone(group Ⅵ), application of acetone & multi-lamellar vesicles (group Ⅶ), & application of acetone & vehicle(group Ⅷ) for the
parafilm-occluded groups. Permeability barrier & water content were determined respectively by measuring transepidermal water loss with evaporimeter and capacitance with corneometer. Each parameter was determined 10 minutes, 1, 3, 6, & 24 hours
after
acetone or D/W treatment for the air-exposed groups & 10 minutes, 1, 6, & 24 hours for the parafilm-occluded groups. The morphologic changes of stratum corneum lipids were determined 1 hour & 24 hours after acetone or D/W treatment by oil red 0
stain.
@ES The results were as follows.
@EN 1. For the air-exposed groups, the value of transepidermal water loss was significantly increased and the value of capacitance was significantly decreased in group Ⅱ, Ⅲ, & Ⅳ, compared to the values in group I in time sequence(P<0.01).
Group
Ⅲ
had an accelerated effect in the recovery of transepidermal water loss & capacitance than group Ⅱ(P<0.05), but the difference in the recovery between group Ⅲ&Ⅳwas not remarkable.
2. For the parafilm-occluded groups, group Ⅵ, Ⅶ & Ⅷ had an increased transepidermal water loss & a decreased capacitance in comparison with group V in time sequence(P<0.01). Group Ⅶshowed a significant effect in the recovery of
transepidermal
water
loss & capacitance than group Ⅵ& Ⅷ(P<0.01).
3. In comparison between air-exposed & parafilm-occluded groups, the occlusion had a significant increase in transepidermal water loss(group Ⅱ& Ⅵ, group Ⅲ & Ⅶ, and group Ⅳ&Ⅷ)(P<0.001). The differences in the values of capacitance between
group
Ⅱ&Ⅵ, and group Ⅲ&Ⅶwere insignificant, but group Ⅷ showed a significantly decreased capacitance than group Ⅳ(P<0.001).
4. A significant negative correlation between transepidermal water loss & capacitance was shown in group Ⅱ, Ⅲ&Ⅳ for the air-exposed groups(rii=-0.94, riii=-0.95 & riv=-0.91 ; P<0.001) and group vii & viii for the parafilm-occluded
groups(rvii=-0.81
& rviii=-0.75 ; P<0.001).
5. Oil red 0 staining of frozen tissues showed loss of lipids 1 hour after acetone treatment & lipids was recovered within 24 hours in group ii, but not in group vi. Application of multi-lamellar vesicles & vehicles after acetone treatment(group
iii &
iv) showed less staining than in control group I in 1 hour and almost staining of control group in 24 hours but more staining under occlusion (group vii & viii) than in control group V in either 1 hour or 24 hours.
Taken together, these results suggest that lamellar structure consisted of stratum corneum lipids may play a role in the permeability barrier as well as in the water content on the disruption of barrier.
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KEYWORD
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